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KMID : 0620920080400030313
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Experimental & Molecular Medicine
2008 Volume.40 No. 3 p.313 ~ p.319
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Effect of xanthohumol on melanogenesis in B16 melanoma cells
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Koo Jeung-Hyun
Choi Il-Whan
Kim Hyung-Tae
Kim Han-Uk
Jung Sung-Hoo
Kwon Kang-Beom
Park Byung-Hyun
Yoon Ha-Yong
Park Jin-Woo
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Abstract
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Xanthohumol (XH), the principal prenylflavonoid of the hop plant (Humulus lupulus L.), dose-dependently inhibited isobutylmethylxanthine (IBMX)-induced melanogenesis in B16 melanoma cells, with little cytotoxicity at the effective concentrations. Decreased melanin content was accompanied by reduced tyrosinase enzyme activity, protein and mRNA expression. The levels of tyrosinase-related protein 1 and 2 mRNAs were decreased by XH. XH also inhibited α-melanocyte stimulating hormone- or forskolin-induced increases in melanogenesis, suggesting an action on the cAMP-dependent melanogenic pathway. XH downregulated the protein and mRNA expression of microphthalmia- associated transcription factor (MITF), a master transcriptional regulator of key melanogenic enzymes. These results suggest that XH might act as a hypo-pigmenting agent through the downregulation of MITF in the cAMP-dependent melanogenic pathway.
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KEYWORD
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cell differentiation, melanins, melanocytes, microphthalmia-associated transcription factor, monophenol monooxygenase, xanthohumol
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